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June 22, 2004Acta Crystallographica Section D Biological Crystallography

Crystallization and preliminary crystallographic study of the functional form of theBacillus thuringiensismosquito-larvicidal Cry4Aa mutant toxin

Panadda Boonserm Chanan Angsuthanasombat Julien Lescar

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Abstract

The 65 kDa functional form of the mosquito-larvicidal Cry4Aa-R235Q mutant toxin has been crystallized. The crystals belong to space group C222(1), with unit-cell parameters a = 91.2, b = 202.1, c = 98.7 A, and contain one molecule per asymmetric unit. The crystals diffract to approximately 2.9 A using synchrotron radiation and a complete native data set has been collected. The structure has been solved using a molecular-replacement method with the Cry4Ba toxin protein as a search model.

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Boonserm et al. (2004) studied this question.

synapsesocial.com/papers/6aae49cd50dfceb7fdf22441 https://doi.org/10.1107/s0907444904011205

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