What question did this study set out to answer?

To resolve the structural basis and conformational dynamics of riboflavin kinase catalytic activity in the bifunctional FAD synthetase from Corynebacterium ammoniagenes.

November 26, 2015Acta Crystallographica Section D Biological CrystallographyOpen Access

Structural insights into the synthesis of FMN in prokaryotic organisms

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Authors

BHBéatriz HerguedasUniversidad de Zaragoza ILIsaías LansMunicipality of Medellín MSMaria Sebastian-ValverdeIcahn School of Medicine at Mount Sinai

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Implication

Structural analysis reveals ligand-induced conformational shifts in Corynebacterium ammoniagenes riboflavin kinase, suggesting how catalytic remodeling drives flavin synthesis.

Key Points

To resolve the structural basis and conformational dynamics of riboflavin kinase catalytic activity in the bifunctional FAD synthetase from Corynebacterium ammoniagenes.
Determined crystal structures of the isolated riboflavin kinase module of CaFADS bound to reaction products FMN and ADP.
Combined molecular-dynamics simulations, binding assays, and kinetic assays evaluating substrate inhibition.
Binding of FMN and ADP triggered extensive structural rearrangements across substantial portions of the riboflavin kinase module.
Molecular-dynamics simulations and substrate inhibition profiling established the sequence of conformational transitions throughout the catalytic cycle and their role within the full-length oligomer.

Cite This Study

Herguedas et al. (2015) studied this question.

synapsesocial.com/papers/6aaf77b38976e698ef351330 https://doi.org/10.1107/s1399004715019641

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Implication

Structural analysis reveals ligand-induced conformational shifts in Corynebacterium ammoniagenes riboflavin kinase, suggesting how catalytic remodeling drives flavin synthesis.

Structural insights into the synthesis of FMN in prokaryotic organisms

Discussion

Member takes

Implication

Key Points

Cite This Study

Structural insights into the synthesis of FMN in prokaryotic organisms

Discussion

Member takes

Implication

Key Points

Cite This Study

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