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Potato leafhopper, Empoasca fabae (Harris), eggs were revealed in situ in Solanum leaves cleared by boiling for 3 minutes in a solution containing one part each of 85% lactic acid, phenol, and distilled water, and two parts of glycerin. Coagulated egg proteins made eggs apparent and they were counted with sub-lighting at 7x magnification under a binocular microscope. Periodic egg counts clearly indicate selective placement of eggs among various Solanum clones and a positive correlation with intensity of nymphal infestation.
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Carlson et al. (1962) studied this question.
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