Inductively coupled plasma mass spectrometry to identify protein drug targets from whole cell systemsElectronic supplementary information (ESI) available: full details of laser ablation ICP-MS and QTOF operating conditions, further experimental details for peptide separation and peptide sequencing maps for OmpA. See http://www.rsc.org/suppdata/cc/b1/b108418f/

Proteins from cisplatin treated bacterial cells were partially separated by 1D polyacrylamide gel electrophoresis and analysed by laser ablation inductively coupled plasma mass spectrometry; using peptide fingerprinting methods central to proteomics, the band containing the highest levels of platinum was found to contain outer membrane protein A, which may be involved in cisplatin uptake.