In contrast o the abundance of studies conducted on chemically transformed rodent cells, there are only a few studies on human cells transformed by direct treatment in vitro with chemicals. Human cells are rel-atively refractory to transformation by carcinogens (Henry 1947; Renwick and Drasan 1976). However, the nontumorigenic human osteogenic sarcoma cell line, HOS TE85 clone 5 (McAllister et al. 1971), after pro-longed treatment in vitro with N-methyl-N'-nitro-N-ni-trosoguanidine (MNNG) by Rhim et al. (1975), gave rise to a culture of morphologically altered cells that were tumorigenic in nude mice (MNNG-HOS). Specific on-cogene sequences termed met were isolated by molec-ular cloning of human DNA from mouse cells trans-formed with MNNG-HOS cell DNA (Cooper et al. 1984). Our studies showed that the met oncogene is contained within 25-35 kb of human DNA (Cooper et al. 1984) and a portion of this was mapped to human chromosome 7 using somatic ell hybrid panels (Coop-er et al. 1984) and to 7q21-q31 by in situ hybridization analysis (Dean et al. 1985). From partial nucleotide se-quence analysis, this region of the met oncogene is sub-stantially homologous to the tyrosine kinase gene fam-ily, including the human insulin receptor and the murine v-abl gene (Dean et al. 1985). Moreover, a tight linkage of the met locus has been established with the hereditary genetic disease cystic fibrosis (CF) (White et al. 1985). This finding allowed the cytological as-signment of the CF gene to chromosome 7q21-q31 (Dean et al. 1985).
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Park et al. (1986) studied this question.