What question did this study set out to answer?

To evaluate whether unfractionated heparin protects against septic acute kidney injury by neutralizing cytotoxic extracellular histones and inhibiting apoptosis and inflammation.

December 2, 2020Frontiers in MedicineOpen Access

Heparin Attenuates Histone-Mediated Cytotoxicity in Septic Acute Kidney Injury

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Authors

ZWZiyi WangHarbin University of Science and Technology LWLijun WangGuangzhou University of Chinese Medicine CCChao CaoShanghai Jiao Tong University

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Implication

Preclinical study demonstrates that heparin reduces histone-induced inflammation and apoptosis in septic mice and renal cells, suggesting a protective role against septic acute kidney injury.

Key Points

To evaluate whether unfractionated heparin protects against septic acute kidney injury by neutralizing cytotoxic extracellular histones and inhibiting apoptosis and inflammation.
C57BL mice (n = 16 per group) were randomized to sham surgery, cecal ligation and puncture (CLP)-induced sepsis, or sepsis treated with subcutaneous unfractionated heparin (0.03 IU/g) administered 4 hours post-CLP, with tissue harvesting at 6 hours and 72-hour survival tracking.
Cultured mouse renal tubular epithelial cells were exposed to histones (40 U) with or without lipopolysaccharide (10 μg/ml) and treated concurrently with heparin (25 IU/ml) for 6 hours.
Heparin-treated septic mice showed significantly reduced serum levels of extracellular histone 4, NGAL, KIM-1, TNF-α, and IL-6, reduced renal edema, preserved glomerular structure, and increased 72-hour survival compared to untreated septic mice (P < 0.05).
Heparin intervention significantly suppressed renal and cellular apoptosis markers, including cleaved Caspase-3/Caspase-3 and Bax/Bcl-2 ratios, at both protein and mRNA levels relative to histone-stimulated controls (P < 0.05).

Cite This Study

Wang et al. (2020) studied this question.

synapsesocial.com/papers/6aaf38dcdfdb3477795d213a https://doi.org/10.3389/fmed.2020.586652

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Implication

Preclinical study demonstrates that heparin reduces histone-induced inflammation and apoptosis in septic mice and renal cells, suggesting a protective role against septic acute kidney injury.

Heparin Attenuates Histone-Mediated Cytotoxicity in Septic Acute Kidney Injury

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Implication

Key Points

Cite This Study

Heparin Attenuates Histone-Mediated Cytotoxicity in Septic Acute Kidney Injury

Discussion

Member takes

Implication

Key Points

Cite This Study

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