Abnormal Innervation and Altered Nerve Growth Factor Messenger Ribonucleic Acid Expression in Ureteropelvic Junction Obstruction

No AccessJournal of UrologyHydronephrosis: Research1 Aug 1995Abnormal Innervation and Altered Nerve Growth Factor Messenger Ribonucleic Acid Expression in Ureteropelvic Junction Obstruction Y. Wang, P. Puri, J. Hassan, H. Miyakita, and D.J. Reen Y. WangY. Wang More articles by this author , P. PuriP. Puri More articles by this author , J. HassanJ. Hassan More articles by this author , H. MiyakitaH. Miyakita More articles by this author , and D.J. ReenD.J. Reen More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)67132-5AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail The pathophysiology of ureteropelvic junction obstruction is unknown. Using specific antibodies, we studied specimens from 35 cases of ureteropelvic junction obstruction and 32 of normal ureteropelvic junction by immunohistochemistry using protein gene product 9.5 (a general neuronal marker), S100 (a supporting cell marker), synaptophysin (a neuromuscular junction marker) and nerve growth factor receptor. Nerve growth factor expression was examined at the messenger ribonucleic acid (mRNA) level using reverse transcription-polymerase chain reaction technique in 11 ureteropelvic junction obstruction specimens and 7 controls. The most striking finding was the marked reduction of protein gene product 9.5, synaptophysin and nerve growth factor receptor staining positive nerve fibers in the muscle layers of ureteropelvic junction obstruction compared to the normal ureteropelvic junction. Supporting nerve cell fibers (S100) were preserved in cases of ureteropelvic junction obstruction and normal ureteropelvic junction. A significantly less intense signal for nerve growth factor mRNA was found in the ureteropelvic junction obstruction specimens compared to normal ureteropelvic junction. These findings suggest that defective innervation may have an important role in the pathogenesis of ureteropelvic junction obstruction, and decreased nerve growth factor mRNA expression may be important in the etiology of ureteropelvic junction obstruction. References 1 : Electron microscopy of the upper ureter and the pelvi-ureteric junction. Brit. J. Urol.1968; 40: 37. Google Scholar 2 : Functional obstruction of the ureter and renal pelvis. A histological and electron microscopic study. Brit. J. Urol.1978; 50: 145. Google Scholar 3 : Observations on the ureteropelvic junction. J. Urol.1970; 104: 252. Link, Google Scholar 4 : Idiopathic hydronephrosis: light microscopic features and pathogenesis. Arch. Path. Lab. Med.1985; 109: 1097. Google Scholar 5 : The musculature of the human ureter. Brit. J. 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Google Scholar Children's Research Centre, Our Lady's Hospital for Sick Children, Dublin, Ireland.© 1995 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byWang G, Brenner-Anantharam A, Vaughan E and Herzlinger D (2018) Antagonism of BMP4 Signaling Disrupts Smooth Muscle Investment of the Ureter and Ureteropelvic JunctionJournal of Urology, VOL. 181, NO. 1, (401-407), Online publication date: 1-Jan-2009.Rosen S, Peters C, Chevalier R and Huang W (2018) The Kidney in Congenital Ureteropelvic Junction Obstruction: A Spectrum From Normal to NephrectomyJournal of Urology, VOL. 179, NO. 4, (1257-1263), Online publication date: 1-Apr-2008.Kajbafzadeh A, Payabvash S, Salmasi A, Monajemzadeh M and Tavangar S (2018) Smooth Muscle Cell Apoptosis and Defective Neural Development in Congenital Ureteropelvic Junction ObstructionJournal of Urology, VOL. 176, NO. 2, (718-723), Online publication date: 1-Aug-2006.KIM D, NOH J, JEONG H, KIM M, JEON H and HAN S (2018) ELASTIN CONTENT OF THE RENAL PELVIS AND URETER DETERMINES POST-PYELOPLASTY RECOVERYJournal of Urology, VOL. 173, NO. 3, (962-966), Online publication date: 1-Mar-2005.SOLARI V, PIOTROWSKA A and PURI P (2018) Altered Expression of Interstitial Cells of Cajal in Congenital Ureteropelvic Junction ObstructionJournal of Urology, VOL. 170, NO. 6, (2420-2422), Online publication date: 1-Dec-2003.NEMETH L, O’BRIAIN D and PURI P (2018) DEMONSTRATION OF NEURONAL NETWORKS IN THE HUMAN UPPER URINARY TRACT USING CONFOCAL LASER SCANNING MICROSCOPYJournal of Urology, VOL. 166, NO. 1, (255-258), Online publication date: 1-Jul-2001.Murakumo M, Nonomura K, Yamashita T, Ushiki T, Abe K and Koyanagi T (2018) Structural Changes of Collagen Components and Diminution of Nerves in Congenital Ureteropelvic Junction ObstructionJournal of Urology, VOL. 157, NO. 5, (1963-1968), Online publication date: 1-May-1997. Volume 154Issue 2August 1995Page: 679-683 Advertisement Copyright & Permissions© 1995 by American Urological Association, Inc.MetricsAuthor Information Y. Wang More articles by this author P. Puri More articles by this author J. Hassan More articles by this author H. Miyakita More articles by this author D.J. Reen More articles by this author Expand All Advertisement PDF downloadLoading ...