Key result
Andrographolide increases survival and limits renal damage and inflammation in rabbits with LPS-induced DIC.
Why the study?
Disseminated Intravascular Coagulation (DIC) leads to multiple organ failure and critical illness, and andrographolide's protective effects on DIC were investigated.
Does andrographolide improve survival and reduce inflammation and coagulation abnormalities in rabbits with LPS-induced DIC?
Does andrographolide improve survival and reduce inflammation and coagulation abnormalities in rabbits with LPS-induced DIC?
Andrographolide demonstrated protective effects against LPS-induced DIC in rabbits by improving survival and modulating inflammatory and coagulation pathways.
Hypothesis-generating in rabbit DIC models; leaves open translation to human therapy pending clinical trials.
Background: The occurrence of Disseminated Intravascular Coagulation (DIC) could lead to multiple organ failure, promoting the development of the critically ill patient's condition.Recently pharmacological studies had shown that andrographolide had effects on anti-inflammatory and preventing thrombosis.Materials and Methods: In order to monitor, if andrographolide has protective effects on DIC rabbits, the DIC models were established by infusing lipopolysaccharide (LPS) in 60 mL of saline solution at a rate of 10 mL/kg/h through the rabbitsʼ marginal ear vein over a period of 6 h.The DIC rabbits were divided into 5 groups; low (1.0 mg kgG 1 ), medium (2.0 mg kgG 1 ), high (5.0 mg kgG 1 ) andrographolide groups, LPS (saline solution) group, heparin (500 IU kgG 1 ) group.The normal control group treated with saline solution, which was given neither LPS nor andrographolide before.Survival rate was monitored by recording the deaths within 6 and 24 h after drug treatment and renal function was detected, including Blood Urine Nitrogen (BUN) and tissue section.Moreover, the concentrations of TNF-" and IL-1$ were determined and the activities of ATIII, protein C, PAI-1 and t-PA in serum level were measured, which was collected at 2 and 6 h post-infusion.All data was presented as Mean±Standard Deviation.Survival rate was analyzed by chi-square test.Differences between two groups were analyzed by one way ANOVA analysis.Results: Treatment of andrographolide significantly increased survival rate and reduced renal damage and increased the activities of protein C, the levels of ATIII and t-PA.But the levels of FDP and PAI-1 were decreased and the suppression of TNF-" and IL-1$ secretion could be found as well.Conclusion: All the data suggested that had positive effects in intervention in DIC rabbits and prevented excessive inflammation via inhibiting the level of TNF-" and IL-1$ in serum.Andrographolide could be a potential drug for DIC treatment.
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Chen et al. (2016) studied Disseminated Intravascular Coagulation (DIC). Andrographolide vs. Saline solution (LPS group) and heparin was evaluated on Survival rate within 6 and 24 h. Andrographolide significantly increased survival rate, reduced renal damage, and prevented excessive inflammation in rabbits with LPS-induced DIC.
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