Key result
AAV2 mosaics containing Z34C and targeting antibodies selectively and efficiently transduce MO7e and Jurkat cells.
Why the study?
Certain cell types are refractory to transduction with AAV-based vectors and targeting specific cell types is desirable for gene therapy applications.
AAV2 mosaics incorporating a protein A fragment and targeting antibodies provide a versatile method for specific cell transduction in gene therapy applications.
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Enables antibody-directed AAV targeting of specific cells; leaves open in vivo efficacy, safety, and clinical translation.
Gigout et al. (2005) studied this question. AAV2 mosaics with a protein A fragment (Z34C) inserted into their capsid vs. all-mutant virions was evaluated on infectivity and selective transduction. AAV2 mosaics containing the immunoglobulin-binding Z34C fragment of protein A, combined with targeting antibodies, selectively and efficiently transduced MO7e and Jurkat cells.
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