Monitoring and Development of Pretreatment Method for Perfluorinated Compounds in Fish Samples

An efficient extraction method was developed for the analysis of 16 perfluorinated compounds (PFCs) in fish samples.We compared the recoveries from the Ion-Pairing Extraction (IPE), Liquid-Liquid Extraction (LLE), Solid-Phase Extraction (SPE), IPE + SPE method with varying the injection time of the internal standard.As a result, IPE method with the internal standard before extraction was evaluated as the most effective pretreatment method.The RPM (Revolution Per Munite) and pH in IPE-before method were additionally adjusted and the more efficient pretreatment method was established.The total 33 fish samples including liver and gut samples were collected from Korean markets and analyzed PFCs with developed pretreatment method of this study.Total 16 PFC levels in fish samples ranged from ND to 1.67 ng/g with 100% detection frequency.The average PFCs concentrations of muscle, liver and gut samples from fish were compared and showed the following trend: liver (17.8 ng/g) > gut (13.3 ng/g) > muscle (1.67 ng/g).The PFC levels in fish samples were similar or lower than other available previous results of foreign studies.