Key result
AMI and stable angina are linked to up-regulated neutrophil and mononuclear-macrophage gene expression versus controls.
Why the study?
Expression differences of neutrophil and mononuclear phagocyte related gene mRNAs among AMI, stable angina, and controls were not well characterized to understand their roles in CAD progression.
Does the expression of neutrophil and mononuclear phagocyte related gene mRNAs differ among patients with acute myocardial infarction, stable angina, and controls?
Observational (n=60)
Does the expression of neutrophil and mononuclear phagocyte related gene mRNAs differ among patients with acute myocardial infarction, stable angina, and controls?
Neutrophil and mononuclear-macrophage function-related gene expression is progressively upregulated across stages of coronary artery disease, peaking in acute myocardial infarction.
OBJECTIVE: To investigate expression differences of neutrophil and mononuclear phagocyte related gene mRNAs among acute myocardial infarction (AMI), stable angina (SA) and control groups, and then discuss their expression characteristics in the stable angina pectoris (SAP) and AMI stages of coronary artery disease (CAD). METHODS: Whole Human Genome Oligo Microarrays were applied to assess the differential expression characteristics of neutrophil and mononuclear phagocyte related mRNAs in patients with AMI (n = 20), SA (n = 20) and controls (n = 20). RESULTS: (1) Almost all colony-stimulating factors (CSF) and their receptors related mRNAs was up-regulated in AMI and SA groups compared with the control group, and the expression of granulocyte-macrophage colony stimulating factor receptor (GM-CSFR) and granulocyte colony stimulating factor receptor (G-CSFR) mRNAs in the AMI group was significantly up-regulated compared with the other two groups (P < 0.01). (2) The expression of mRNAs related to monocyte chemoattractant protein-1 (MCP-1), CCR2 (MCP-1 receptor) and CXCR2 (IL-8 receptor) was significantly up-regulated (P < 0.01) in AMI group compared with SA and control groups. IL-8 mRNA expression in the AMI group was clearly higher than the controls (P < 0.05). (3) All mRNAs expression related to opsonic receptors (IgG FcR and C3bR/C4bR) was significantly up-regulated in AMI group compared with SA and control group (P < 0.01), and the SA group showed an upward trend compared with controls. (4) Most pattern recognition receptor (PRR)-related mRNAs expression was up-regulated in AMI group compared with SA and control groups. Most toll-like receptor (TLR) mRNAs expression was significantly up-regulated (P < 0.01) than the SA and control groups; macrophage scavenger receptor (MSR) mRNA was significantly up-regulated in AMI group compared with the control group (P < 0.01), and the SA group showed an upward trend compared with the controls. CONCLUSIONS: The expression of most neutrophil and mononuclear-macrophage function related genes mRNAs was significantly up-regulated by stages during the progression of CAD, suggesting that the adhesive, chemotactic and phagocytic functions of neutrophil and mononuclear-macrophage were strengthened in the occurrence and development of coronary atherosclerosis and AMI. This also showed a stepped upward trend as the disease progressed.
No takes yet. Share an insight, caveat, or question.
Li et al. (2015) conducted an observational in Coronary artery disease (n=60). Coronary artery disease stages (AMI and SA) vs. Controls was evaluated on Expression of neutrophil and mononuclear phagocyte related gene mRNAs (p=<0.01). Acute myocardial infarction and stable angina were associated with significantly up-regulated expression of neutrophil and mononuclear-macrophage related gene mRNAs compared to controls (P<0.01).
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: