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The occurrence of new cardiotonic steroid conjugates in the skin of a tropical toad, Bufo marinus (L.) SCHNEIDER, is reported. These substances were separated by chromatography on Amberlite XAD-4 resin and silica gel, high-performance liquid chromatography, and gel chromatography on Sephadex LH-20. The sulfated bufogenins, marinobufagin 3-sulfate and resibufogenin 3-sulfate, were characterized by degradative and synthetic means. In addition, the structures of two new bufotoxins were elucidated as marinobufagin 3-pimeloylarginine ester and telocinobufagin 3-suberoylarginine ester.
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Shimada et al. (1979) studied this question.
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