The Presence of Virus Neutralizing Antibodies Is Highly Associated with Protection against Virulent Challenge in Domestic Pigs Immunized with ASFV live Attenuated Vaccine Candidates

Figures

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  Figure 1. ELISA titers in sera obtained at the time of challenge in animals immunized with either ASFV-G-Δ9GL/ΔUK or ASFV-G-ΔI177L vaccine stains. Black dots and red dots indicate presence and absence of protection against the challenge, respectively.

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  Figure 2. Neutralization index in sera obtained at the time of challenge in animals immunized with either ASFV-G-Δ9GL/ΔUK or ASFV-G-ΔI177L vaccine stains. The ROC curve was calculated with AUC = 0.7745 and Cut-off < 1.690 with specificity of 95.71% and Sensitivity of 50%. Black dots and red dots indicate presence and absence of protection against the challenge, respectively.

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  Figure 3. Neutralization index (NI) in sera of animals vaccinated with ASFV-G-Δ9GL/ΔUK or ASFV-G-ΔI177L vaccine strains and challenged with virulent field strain ASFV-G. Animals were grouped based on survival (black symbols) or absence of protection (red symbols) after the challenge. Asterisks indicate differences among groups (p < 0.05).

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  Figure 4. Comparison of neutralization index (NI) and anti-ASFV ELISA titers in sera of animals vaccinated with ASFV-G-Δ9GL/ΔUK or ASFV-G-ΔI177L vaccine stains. Pearson correlation coefficient (r) 0.65; 95% of interval 0.5004 to 0.7898; P (two-tailed) 0.0001.

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  Figure 5. Correlation between protection after challenge and Neutralizing Index (NI) in sera of animals vaccinated with ASFV-G-Δ9GL/ΔUK or ASFV-G-ΔI177L vaccine stains. Survival percentage is represented by number of survivals groups described in Table 1. Pearson correlation coefficient r = 0.6841, p < 0.05; 95% confidence interval 0.0368 to 0.9271; P (two-tailed) 0.0421.