Key result
Prolonged PMA exposure downregulates multiple PKC isozymes and enhances spontaneous contraction rates.
Why the study?
The role of protein kinase C isozymes in modulating cardiac functions and contraction rate in neonatal cardiac myocytes was not fully understood.
Population
Neonatal rat cardiac myocytes in culture
Comparison
Prolonged exposure to 0.1-1 nM and 100 nM PMA vs no PMA
Design
Preclinical experimental study
Follow-up
2 days
Authors
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Implicates beta/delta/epsilon PKC in myocyte contractility; hypothesis-generating for isozyme-selective targeting in cardiac disease.
Prolonged PMA exposure in neonatal rat cardiac myocytes alters specific PKC isozymes and enhances contraction rate, suggesting beta, delta, or epsilon PKC isozymes mediate this effect.
Johnson et al. (1995) studied this question. 4-beta phorbol 12-myristate-13-acetate (PMA) was evaluated on PKC isozyme levels and rate of spontaneous contraction. Prolonged exposure to 100 nM PMA for 2 days caused complete down-regulation of alpha and zeta-like PKC, incomplete down-regulation of beta, delta, and epsilon PKC, and enhanced the contraction rate.
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