April 1, 1990Circulation ResearchOpen Access

Phorbol ester and dioctanoylglycerol stimulate membrane association of protein kinase C and have a negative inotropic effect mediated by changes in cytosolic Ca2+ in adult rat cardiac myocytes.

View Full Paper

Ask AI

Bookmark

Population

Left ventricular myocytes from adult rats

Comparison

4 beta-phorbol 12-myristate 13-acetate (3 x 10… vs Control conditions (untreated or 5 mM KCl)

Design

Preclinical

Authors

MCM. C. CapogrossiNational Institutes of Health TKToshihiko KakuNational Institutes of Health CFCharles R. FilburnUniversity of Southern California

Discussion

Member takes

Implication

PKC activation may reduce myocyte contractility via Cai transients; leaves open therapeutic targeting in human HF models.

Structured PICO

Population

Left ventricular myocytes from adult rats

Intervention

4 beta-phorbol 12-myristate 13-acetate (PMA) (3 x 10(-10) and 3 x 10(-7) M) and sn-1,2-dioctanoylglycerol (DiC-8) (10(-5) and 10(-4) M)

Comparator

Control conditions (untreated or 5 mM KCl)

Outcome

Membrane association of protein kinase C (PKC), cytosolic Ca2+ (Cai) homeostasis, and contractile properties (twitch amplitude)surrogate

Activation of PKC by phorbol esters and diacylglycerol analogues induces a negative inotropic effect in adult rat cardiac myocytes by reducing the cytosolic calcium transient.

Cite This Study

Capogrossi et al. (1990) studied this question.

synapsesocial.com/papers/6a7154ebf44fa9f079df2023 https://doi.org/10.1161/01.res.66.4.1143

View Full Paper

Ask AI

Bookmark