Key result
miR-192-5p upregulation suppresses proliferation, migration, and angiogenesis in high-glucose retinal endothelial cells by targeting ELAVL1.
Why the study?
The role and mechanism of miR-192-5p in diabetic retinopathy progression remain unclear.
p-value: p=<0.05
MiR-192-5p attenuates diabetic retinopathy progression in vitro by targeting the ELAVL1/PI3Kδ axis, suggesting a potential therapeutic target.
MiR-192-5p modulation remains preclinical; leaves open whether ELAVL1/PI3Kδ targeting alters diabetic retinopathy progression in vivo.
BACKGROUND: Diabetic retinopathy (DR) is a common complication of diabetes mellitus that poses a threat to adults. MicroRNAs (miRNAs) play a key role in DR progression. However, the role and mechanism of miR-192-5p in DR remain unclear. We aimed to investigate the effect of miR-192-5p on cell proliferation, migration and angiogenesis in DR. METHODS: Expression of miR-192-5p, ELAV-like RNA binding protein 1 (ELAVL1) and phosphoinositide 3-kinase delta (PI3Kδ) in human retinal fibrovascular membrane (FVM) samples and human retinal microvascular endothelial cells (HRMECs) was assessed using RT-qPCR. ELAVL1 and PI3Kδ protein levels were evaluated by Western blot. RIP and dual luciferase reporter assays were performed to confirm the miR-192-5p/ELAVL1/PI3Kδ regulatory networks. Cell proliferation, migration and angiogenesis were assessed by CCK8, transwell and tube formation assays. RESULTS: MiR-192-5p was decreased in FVM samples from DR patients and high glucose (HG)-treated HRMECs. Functionally, overexpressed miR-192-5p inhibited cell proliferation, migration and angiogenesis in HG-treated HRMECs. Mechanically, miR-192-5p directly targeted ELAVL1 and decreased its expression. We further verified that ELAVL1 bound to PI3Kδ and maintained PI3Kδ mRNA stability. Rescue analysis demonstrated that the suppressive effects of HG-treated HRMECs caused by miR-192-5p up-regulation were overturned by overexpressed ELAVL1 or PI3Kδ. CONCLUSION: MiR-192-5p attenuates DR progression by targeting ELAVL1 and reducing PI3Kδ expression, suggesting a biomarker for the treatment of DR.
No takes yet. Share an insight, caveat, or question.
Fu et al. (2023) studied Diabetic retinopathy (n=40). miR-192-5p upregulation vs. Negative control / High glucose alone was evaluated on Microvascular endothelial cell proliferation, migration, and angiogenesis (p=<0.05). Upregulation of miR-192-5p significantly suppressed cell proliferation, migration, and angiogenesis in high glucose-treated human retinal microvascular endothelial cells by targeting ELAVL1.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: