Key result
Elevated lncRNA AF085935 and GPC3 protein detect HCC, with the lncRNA yielding ~96% sensitivity.
Why the study?
The molecular mechanisms of hepatocellular carcinoma are poorly understood, and the role of long non-coding RNAs and Glypican3 in HCC pathogenesis requires further assessment.
Do LncRNA AF085935 gene expression and GPC3 protein levels serve as diagnostic and prognostic biomarkers in patients with hepatocellular carcinoma?
Case-Control (n=144)
No
Do LncRNA AF085935 gene expression and GPC3 protein levels serve as diagnostic and prognostic biomarkers in patients with hepatocellular carcinoma?
Effect estimate: AUC 0.813 (95% CI 0.739-0.888)
p-value: p=<0.001
LncRNA AF085935 gene expression and GPC3 levels are promising diagnostic and prognostic biomarkers for hepatocellular carcinoma.
Elevated lncRNA AF085935 and GPC3 support HCC detection and prognosis; leaves open clinical adoption pending prospective validation.
Background Hepatocellular carcinoma (HCC) is one of the main cancers in the world with a high mortality rate. The molecular mechanisms of HCC are poorly understood. Long non-coding RNAs (lncRNAs) have a role in HCC pathogenesis. Glypican3 (GPC3) is a cell surface oncofetal proteoglycan that is expressed in HCC, and its overexpression predicts a poorer prognosis. We aimed to assess the levels of alfa fetoprotein (AFP), lncRNA AF085935 gene expression, and GPC3 protein in HCC patients. Patients and methods The patients were classified into three groups: HCC group, cirrhotic group, and healthy control group. For all groups, we performed clinical examinations, laboratory investigations, and imaging. The levels of AFP, GPC3 protein, and LncRNA gene expression were estimated. A statistical analysis was done. Results Levels of GPC3 and LncRNA gene expression were significantly higher in the HCC group versus other groups. LncRNA gene and GPC3 levels are excellent for the detection of HCC with a sensitivity of 96% and 87%, respectively. Specificity was 81% and 64%, respectively. Linear regression analysis showed that LncRNA gene expression and GPC3 protein are significant predictors for HCC (p = 0 and p = 0.001, respectively). Log rank analysis based on GPC3 and LncRNA gene expression levels in HCC patients showed that high expression of GPC3 and LncRNA is associated with shorter overall survival than those with low expressions (p value < 0.001). Conclusion In our study, LncRNA gene expression and GPC3 levels are good diagnostic and prognostic biomarkers for HCC patients.
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Ramadan et al. (2022) conducted a case-control in Hepatocellular carcinoma (n=144). lncRNA AF085935 and GPC3 protein levels vs. Cirrhosis and healthy controls was evaluated on Diagnostic accuracy (AUC) of lncRNA AF085935 for detecting HCC (AUC 0.813, 95% CI 0.739-0.888, p=<0.001). Elevated plasma levels of lncRNA AF085935 and GPC3 protein accurately detected hepatocellular carcinoma, with lncRNA AF085935 demonstrating 96% sensitivity and 81% specificity.
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