Endogenous Gene Regulation as a Predicted Main Function of Type I-E CRISPR/Cas System in E. coli

Figure 1. (A) Overall organization of the analyzed CRISPR/Cas locus generated by [42]. (B) Schematic organization of repeats and spacers within the CRISPR array. (C) Scheme of in silico analysis of canonical vs. non-canonical activity.

Figure 2. Normalized probability distributions of alignment scores for (i) E. coli genome—black solid curve, (ii) the genomes of all sequenced E. coli infecting bacteriophages—gray solid curve, (iii) randomized E. coli genome background—dashed black curve, (iv) randomized bacteriophage genome background—dashed grey curve.

Figure 3. (A) Phylogenetic distribution of I-E E. coli CRISPR spacers throughout the domain of bacteria vs. E. coli species; (B) Phylogenetic distribution of I-E E. coli CRISPR spacers and their putative genomic targets across the NCBI representative set of prokaryotic genomes. For CRISPR array, the sum of hits obtained for all 12 spacers (with three standard deviations) is shown (white diamond). For CRISPR spacer genomic targets (19 targets per spacer), the mean number of hits, summed over 12 spacers, with three standard deviations, is shown (purple diamond).

Figure 4. (A) SAC values associated with hits located in coding and (B) intergenic regions of E. coli vs. randomized E. coli background; (C) SAC values associated with hits located in divergent, (D) convergent and (E) upstream intergenic regions, preceded by schematic representation of the intergenic region classification; On each plot, SAC values are shown as a function of the growing number of hits per spacer, with confidence bound estimates provided for the SAC values associated with randomized background.

Figure 5. (A) Schematic representation of the functional classification of hits associated with coding regions, based on the DNA strand that is being targeted, and (B) the interference RnpC; (C) The plot showing the difference between SAC values associated with targets classified as RNA− and RNA+ category.

Figure 6. (A) The plot showing the difference between SAC and (B) SAS values associated with hits on the reverse and direct DNA strand of the E. coli genome shown as a function of the increasing number of hits per chain.

Figure 7. (A) The PAM-wheel of 3nt-long statistically overrepresented motifs flanking the theoretical and (B) the actual start of the alignment on the targeted strand, in the 3′–5′ direction. The percentages are shown as a function of the associated Z scores [(actual motif counts—sum of all overrepresented motif counts)/sum of all overrepresented motif counts)].