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An HPTLC method for identification and determination of 12 synthetic dyes (Amaranth, Allura red, Brilliant black BN, Brilliant blue E, Carmoisine, Erythrosine, Indigotine, Patent blue V, Ponceau 4R, Quinoline yellow, Sunset yellow, Tartazine) has been developed. Amberlite XAD-2 was used as a stationary phase for column chromatography. Elution was performed with various acidified alcohols (i-propanol, n-propanol, n-butanol) and recoveries between 81.5 - 100.2% were obtained. The low limit of detection is in the range of 4 – 10 ng. The method was tested on commercial products as: carbonated soda, candies, chewing gum, identical natural flavours, etc.
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Rizova et al. (1995) studied this question.
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