Determination of Inorganic Selenium Species in Dietary Supplements by Hyphenated Analytical System HPLC-HG-AAS

Construction and optimisation of a hyphenated analytical system for high performance liquid chromatography with hydride generation atomic absorption spectrometry detection system are described together with results of its use for investigation of food products. The use of anion-exchange chromatographic column permitted separation of inorganic selenium species Se(IV) and Se(VI) at the limit of detection of 10 ng mL −1 for both species. The analytical system was used for determination of selenium species in dietary supplements available on the Polish market. The content of inorganic selenium species was found not to exceed 50% of its total content, and in the majority of products, the dominant species was Se(IV). In the majority of products, the total contents of selenium were close to those declared by the producers.