Fecal shedding of a highly cell-culture-adapted porcine epidemic diarrhea virus after oral inoculation in pigs

After oral inoculation with a highly cell-culture-adapted strain of porcine epidemic diarrhea virus, shedding was detected by reverse transcription-polymerase chain reaction and restriction fragment length polymorphism for up to 6 days in 3-day-old piglets, 9 days in 2-week-old pigs, and 3 days in late-term pregnant sows.