Chemical composition and molecular weight of yeast tannase.

The molecular weight of the yeast tannase [E.C. 3.1.1.20,tannin acyl-hydrolase] of Candida sp. was determined to be 250,000 by gel filtration on Sephadex G-200 .The enzyme was dissociable into two identical subunits with molecular weight of 120,000 on SDS-polyacrylamide gel electrophoresis.The amino acid analysis revealed that the enzyme consisted of 786 amino acid residues per protein molecule.The polypeptide moiety of the enzyme was 38 by the Lowry-Folin reaction and 35% by the amino acid analysis.The enzyme contained 62% neutral sugars, which were identified as mannose and galactose on cellulose thin-layer chromatogram and 2.2% hexosamines.