What question did this study set out to answer?

To elucidate the molecular mechanism by which rapamycin inhibits IGF-1-stimulated lamellipodia formation, cytoskeleton reorganization, and cell motility.

October 12, 2010Journal of Biological ChemistryOpen Access

Rapamycin Inhibits Cytoskeleton Reorganization and Cell Motility by Suppressing RhoA Expression and Activity

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Authors

LLLei LiuBrigham and Women's Hospital YLYan LuoNanjing University of Chinese Medicine LCLong ChenSoochow University

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Implication

Experimental study demonstrates rapamycin inhibits motility in mammalian cells by suppressing RhoA via mTORC1, indicating a key signaling axis for cytoskeleton reorganization.

Key Points

To elucidate the molecular mechanism by which rapamycin inhibits IGF-1-stimulated lamellipodia formation, cytoskeleton reorganization, and cell motility.
Assessed protein synthesis and enzymatic activity of RhoA, Cdc42, and Rac1 following rapamycin treatment and siRNA-mediated silencing of raptor (mTORC1) or rictor (mTORC2).
Transfected cells with constitutively active or mutant forms of S6K1, 4E-BP1, RhoA, Cdc42, and Rac1 to evaluate rescue of cell motility and lamellipodia formation.
Rapamycin reduced both protein synthesis and activity of RhoA, Cdc42, and Rac1, with mTORC1 disruption selectively mimicking the suppression of GTPase protein expression.
Modulating S6K1 and 4E-BP1 demonstrated that both downstream mTORC1 signaling branches regulate the translation and expression of Rho family GTPases.
Expression of constitutively active RhoA, but not Cdc42 or Rac1, rescued IGF-1-stimulated lamellipodia formation and cell migration from rapamycin inhibition.

Cite This Study

Liu et al. (2010) studied this question.

synapsesocial.com/papers/6ab084ca65e682e32af32b1b https://doi.org/10.1074/jbc.m110.141168

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Implication

Experimental study demonstrates rapamycin inhibits motility in mammalian cells by suppressing RhoA via mTORC1, indicating a key signaling axis for cytoskeleton reorganization.

Rapamycin Inhibits Cytoskeleton Reorganization and Cell Motility by Suppressing RhoA Expression and Activity

Discussion

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Implication

Key Points

Cite This Study

Rapamycin Inhibits Cytoskeleton Reorganization and Cell Motility by Suppressing RhoA Expression and Activity

Discussion

Member takes

Implication

Key Points

Cite This Study

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