Key result
Bradykinin promotes mesangial cell proliferation and ECM secretion alone but inhibits them alongside PDGF.
Why the study?
The mechanism by which bradykinin delays glomerulosclerosis through effects on mesangial cell proliferation and extracellular matrix secretion remains unclear.
Does bradykinin affect PDGF-induced proliferation and ECM secretion in renal mesangial cells?
Does bradykinin affect PDGF-induced proliferation and ECM secretion in renal mesangial cells?
Bradykinin exhibits a bidirectional effect on mesangial cell proliferation and ECM secretion, promoting them alone but inhibiting them when combined with PDGF, likely via the ERK1/2 pathway.
Hypothesis-generating for bradykinin in glomerular disease; requires validation before clinical consideration.
Recent studies have found that bradykinin (BK) plays a role in delaying glomerulosclerosis, although the mechanism of this phenomenon remains unclear. Mesangial cell proliferation (MCP) and extracellular matrix (ECM) secretion are important mechanisms for glomerulosclerosis. This study investigated the impact of BK on the platelet-derived growth factor (PDGF)-induced proliferation of mesangial cells, and evaluated its correlations with the extracellular signal-related kinase (ERK) signaling pathway. The results showed that on its own, 10-1000 mg/L BK promoted MCP and ECM secretion and induced ERK phosphorylation. However, BK administration after PDGF pre-incubation inhibited PDGF-induced MCP, ECM secretion, and ERK phosphorylation. The BK B2 receptor-specific antagonist, HOE-140, and tyrosine phosphatase inhibitor (OV) effectively blocked the function of BK. In summary, these results demonstrated that BK has a bidirectional effect on MCP and ECM secretion: when used alone, it promoted effects on these phenomena, but these effects were inhibited when combined with PDGF. This suggests that the role of BK might be achieved through inhibiting activation of the PDGF-induced ERK1/2 pathway.
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Liu et al. (2014) studied Glomerulosclerosis. Bradykinin vs. Control / PDGF alone was evaluated on Mesangial cell proliferation and extracellular matrix secretion. Bradykinin exhibited a bidirectional effect, promoting mesangial cell proliferation and extracellular matrix secretion when used alone, but inhibiting these effects when combined with PDGF.
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