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THE etiological agent of the so-called air-sac infection in poultry has been shown by Markham and Wong (1952) to be an avian pleuropneumonia-like organism (PPLO). Van Herick and Eaton (1945) were the first to demonstrate hemagglutination of PPLO isolated from chicken embryos. Jungherr et al. (1954) applied the hemagglutination-inhibition test for the detection of PPLO infection in chickens known as chronic respiratory disease (CRD). It was then shown by Fahey (1954) that the hemagglutination-inhibition test could also be used to demonstrate the disease in turkeys known as infectious sinusitis (IST). Adler and Yamamoto (1956) developed a new culture method for production of a satisfactory antigen to detect PPLO in CRD and IST. Gainforte et al. (1955) subjected 7 strains of PPLO isolated from CRD and IST to an antigenic analysis. The tube agglutination and cross-agglutination tests indicated all strains were antigenically identical. The detection of 4 distinct antigenic types of …
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Noel et al. (1962) studied this question.
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