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= 3 per treatment). Elemental concentrations in feed, larvae, and frass were quantified using ICP-OES to determine the enrichment patterns. The results showed calcium exhibited a substrate-driven response, with strong enrichment in frass (up to 12.15%) and moderate accumulation in larvae. Manganese and zinc showed selective larval uptake at lower inclusion levels, while higher shell inclusion shifted these elements toward frass enrichment. Aluminium, iron, boron, and barium were largely excluded from larval biomass and concentrated in frass. Across both shrimp- and crab-based treatments, frass consistently showed a higher enrichment ratio than larvae for most elements. These findings suggest that BSF bioconversion produces safe, mineral-low larval protein while simultaneously generating mineral-enriched frass for potential use as a slowly mobilized organic soil amendment.
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Upadhyaya et al. (2026) studied this question.
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