Key result
Semi-automatic Optical Heartbeat Analysis provides a novel movement detection algorithm to analyze preclinical animal hearts.
Why the study?
A method was needed to analyze high speed optical recordings of small hearts from Drosophila, zebrafish, and embryonic mice to quantify cardiac function.
SOHA provides a semi-automated, high-throughput method for analyzing cardiac function and arrhythmicity in small animal models like Drosophila, zebrafish, and embryonic mice.
SOHA enables detailed cardiac phenotyping in model organisms; leaves open validation and translation to mammalian or human applications.
We have developed a method for analyzing high speed optical recordings from Drosophila, zebrafish and embryonic mouse hearts (Fink, et. al., 2009). Our Semi-automatic Optical Heartbeat Analysis (SOHA) uses a novel movement detection algorithm that is able to detect cardiac movements associated with individual contractile and relaxation events. The program provides a host of physiologically relevant readouts including systolic and diastolic intervals, heart rate, as well as qualitative and quantitative measures of heartbeat arrhythmicity. The program also calculates heart diameter measurements during both diastole and systole from which fractional shortening and fractional area changes are calculated. Output is provided as a digital file compatible with most spreadsheet programs. Measurements are made for every heartbeat in a record increasing the statistical power of the output. We demonstrate each of the steps where user input is required and show the application of our methodology to the analysis of heart function in all three genetically tractable heart models.
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Ocorr et al. (2009) studied Drosophila, zebrafish and embryonic mouse hearts. Semi-automatic Optical Heartbeat Analysis (SOHA) was evaluated on Physiologically relevant readouts including systolic and diastolic intervals, heart rate, and heartbeat arrhythmicity. Semi-automatic Optical Heartbeat Analysis (SOHA) provides a novel movement detection algorithm to analyze cardiac movements and physiological readouts in Drosophila, zebrafish, and embryonic mouse hearts.
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