A variety of amines and peptides were evaluated in an in vitro system for their effect on the release of FSH which was assayed by the HCG-augmentation method of Steelman and Pohley. Of the monoamines tested, both epinephrine and norepinephrine enhanced release of FSH at high doses (⋝10 /μg/ml of incubation medium), although epinephrine appeared to be the more potent of the two. Dopamine was ineffective except at the higher doses (⋝10 μg/ml), which inactivated the FSH released. Histamine was also active at higher doses (⋝10 μg/ml), but serotonin failed to alter FSH release at all doses tested. Putrescine, a diamine, was virtually inactive except at the highest dose (100 μg/ml), which had a weak FSH-releasing effect, whereas cadaverine, another diamine, was active at doses of 10 μg/ml or more and produced a more markedeffect. Of the polyamines tested, spermidine was highly active at doses of 10 μg/ml or more, whereas spermine was without effect at all doses tested. Arginine-vasopressin at a very high concentration (1000 mU/ml) also induced release of FSH, but 2 other neurohypophyseal hormones, lysine-vasopressin and oxytocin, were ineffective. Protamine, another peptide, had an FSH-releasing effect which was very marked at the highest dose (100 μg/ml). The effective doses of the above-mentioned biogenic amines and peptides had themselves no effect on the assay of FSH, and failed to augment the effect of FRF except for spermidine and arginine vasopressin, which produced further release of FSH when combined with FRF. (Endocrinology85: 815, 1969)
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KAMBERI et al. (1969) studied this question.