Atmospheric nitrogen is fixed in the root zone of rice plants ( Oryza sativa L.). Acetylene‐ethylene assay showed that bacteria, rather than the root tissues, do the fixation. Although the root tissues produce a trace of ethylene, they cannot reduce acetylene to ethylene. The apparent K m (Michaelis constant) of acetylene was much higher than values from known nitrogenfixing bacteria.
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Yoshida et al. (1971) studied this question.