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Background and Aim: This study compared the marginal adaptation of GuttaFlow 2, GuttaFlow Bioseal, and AH Plus in the apical third of human single-canal teeth obturated by the single-cone technique. Materials and Methods:This in vitro study evaluated 51 eligible extracted single-canal maxillary incisors.The teeth underwent biomechanical preparation with BioRaCe rotary system to #40/4%, and were then randomly divided into 3 experimental groups (n=17) of GuttaFlow 2, GuttaFlow Bioseal, and AH Plus.The canals were rinsed with 17% EDTA for 1 minute, 5.25% NaOCl for 30 seconds, and 2 mL of distilled water for smear layer removal, and were obturated with the single-cone obturation technique.The roots were then transversely sectioned at 3 and 6 mm from the apex, and the gap at the root filling material-canal wall interface was measured under a scanning electron microscope (SEM).Data were analyzed with the Kruskal-Wallis and Mann-Whitney U tests (alpha=0.05).Results: At 3 mm distance from the apex, the mean gap size in the GuttaFlow Bioseal group (12.65±4.919µm) was significantly larger than that in GuttaFlow (7.7±2.969µm) and AH Plus (6.89±4.58µm) groups (P<0.05),but no significant difference was detected at 6 mm from the apex among the three groups (P=0.121).Conclusion: Under the limitations of this in vitro study, the results indicated that GuttaFlow Bioseal had poorer marginal adaptation than AH Plus and GuttaFlow at 3 mm from the apex; however, marginal adaptation of sealers was not significantly different at 6 mm from the apex.
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Abbasnejad et al. (2026) studied this question.
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