Single-cell transcriptomics of peripheral blood mononuclear cells indicates impaired immune and inflammatory responses in alcohol-associated hepatitis

Fig. 1.. Data analysis flowchart and cell type annotation. A) Analysis flowchart for single-cell data. B) UMAP plot of cell clusters from integrated scRNA-seq samples colored by cell types. C) Histogram of cell proportion from each participant, colored by cell types.

Fig. 2.. Expression levels of select genes and KEGG enrichment analysis. Expression levels of select genes from A) monocytes, B cells and dendritic cells; and B) T cells, NKT cells and NK cells shown as violin plot. Red represents AH samples and green represents healthy control. C) Network visualization of KEGG enrichment analysis of top 308 DEGs by cell type similarity and regulation directionality of gene expression. Cell types were categorized to 4 groups: group 1 = CD4, CD8; group 2 = The 3 types of monocytes + DCs; group 3 = NK, NKT; group 4 = B cells. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Fig. 3.. Pseudotime analysis of monocytes. A) Heatmap of most variable genes in monocytes grouped by clusters. B) Pseudotime analysis of monocytes determined by Slingshot. C) Heatmap of expression levels of top 87 genes that most expressed differently across pseudotime and across conditions by lineage 1 (full lineage, goes from CM (C2, C3, C0) to IM(C1) then to NCM(C4)). X-axis represents pseudotime from left to right.

Fig. 4.. Mechanism illustration of the inflammation and immune responses in AH. Illustration of the inflammation and immune responses in peripheral T-cells, NK cells, and monocytes from AH patients. Created with BioRender.com.