A rapid and simple high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous quantitative estimation of the biologically active diterpenoids, 14-deoxy-11,12-didehydroandrographolide, andrographolide, neoandrographolide and andrographiside in Andrographis paniculata. The assay combines the isolation and separation of andrographolide derivatives on silica gel 60 F254 HPTLC plates with spot visualization and scanning at 540 nm. Methanol was found to be the most appropriate solvent for the exhaustive extraction of andrographolide derivatives.
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Saxena et al. (2000) studied this question.
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