Key result
New HPTLC method accurately estimates teriflunomide levels in human plasma.
Why the study?
A simple and economical HPTLC method for estimation of Teriflunomide in human plasma was needed for therapeutic drug monitoring.
A simple, specific, accurate, and economical HPTLC method was successfully developed and validated for estimating Teriflunomide in human plasma.
Answers are grounded in this paper and cite their sources.
Validated HPTLC assay supports teriflunomide plasma quantification; leaves open clinical adoption pending further validation.
Objective: Teriflunomide is used for the treatment of multiple sclerosis and is available in 7mg and 14mg tablets. This study aimed to develop and validatea simple and economical HPTLC method for the estimation of Teriflunomide in human plasma. Methods: HPTLC method was developed using toluene: ethyl acetate: acetic acid as the mobile phase and the stationary phase was a TLC plate precoated with silica gel 60 F254. The detection wavelength set was 294nm. The sample preparation involved a simple protein precipitation technique with Acetonitrile as a protein precipitating agent, the internal standard selected was Rilpivirine.The validationwas carried out as per bio-analytical method guidelines. Results: The Rf value for Teriflunomide was found to be 0.46±0.04. The linearity range was observed from 10-60 µg/ml with a regression coefficient value of 0.9819. The developed method was validated for various parameters like specificity, linearity, accuracy, precision, recovery, and stability. Conclusion: The developed method is simple, specific, accurate, and economical for the estimation of Teriflunomide in human plasma.
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GURAV et al. (2022) studied this question. Teriflunomide was evaluated on Linearity range and regression coefficient. A simple, specific, accurate, and economical HPTLC method was developed and validated for the estimation of Teriflunomide in human plasma with a linearity range of 10-60 µg/ml.
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