A simple and reliable RP-HPLC method for the separation and quantitative determination of p-phenylenediamine, p-aminophenol, m-aminophenol, resorcinol, and 2,6-diaminopyridine in commercial oxidation hair dyes was developed. The separation was carried out on a μ-Bondapak C18 column with methanol-aqueous solution containing 0.1% triethylamine and 0.02 mol l−1 NH4Ac (pH = 5.20) (10:90, vol./vol.) as the mobile phase. Without a complex extraction procedure, the method is sufficiently rapid and accurate for routine analyses. Two kinds of commercial hair dye samples were determined successfully.
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Shao et al. (2001) studied this question.
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