Key result
Voluntary wheel running increases intestinal LDL-R and PCSK9 gene expression in rats across all diets.
Why the study?
The effects of exercise training on intestinal membrane receptors involved in transintestinal cholesterol excretion (TICE) were unclear in intact and ovariectomized rats.
Does voluntary wheel running increase intestinal gene expression of LDL-R and PCSK9 in intact and ovariectomized rats?
Does voluntary wheel running increase intestinal gene expression of LDL-R and PCSK9 in intact and ovariectomized rats?
Voluntary wheel running upregulates intestinal LDL-R and PCSK9 gene expression in rats, suggesting exercise may enhance transintestinal cholesterol excretion.
Hypothesis-generating for exercise effects on transintestinal cholesterol excretion in rats; human studies required before any clinical consideration.
Transintestinal cholesterol excretion (TICE) is known as an alternate non-biliary route of cholesterol excretion from the body. The aim of this study was to determine whether exercise training has effects on intestinal membrane receptors involved in TICE in intact and ovariectomized (Ovx) rats. Sprague-Dawley rats were first divided into 4 groups: Sham operated and Ovx rats fed a standard diet (Sham-SD; Ovx-SD), or a high cholesterol diet (Sham-Chol; Ovx-Chol). These 4 groups were subsequently subdivided into either sedentary or voluntary wheel running groups for 6 weeks. The cholesterol diet resulted in increased hepatic cholesterol accumulation (p< 0.001) in both Sham and Ovx rats. Exercise training increased (p < 0.01) transcripts of intestinal low density lipoprotein receptor (LDL-R) and proprotein convertase subtilisin/kexin type 9 (PCSK9), which are involved in trans-intestinal cholesterol uptake from circulation, in both Sham and Ovx rats compared to rats remaining sedentary in all diet conditions. The up-regulation of intestinal gene expression of LDL-R and PCSK9 following voluntary wheel running in intact and Ovx rats suggests that exercise training may contribute to elimination of cholesterol through the TICE pathway.
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Farahnak et al. (2018) studied Cholesterol metabolism (n=65). Voluntary wheel running (exercise training) vs. Sedentary condition was evaluated on Intestinal gene expression of LDL-R and PCSK9 (p=<0.01). Voluntary wheel running significantly increased intestinal gene expression of LDL-R and PCSK9 (p < 0.01) in both intact and ovariectomized rats across all diet conditions.
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