Studies on Male Infertility

We made qualitative and quantitative analyses of free amino acids in human ejaculates with an automatic analyser.We used two different buffers: sodium citrate and lithium citrate.Results were as follows:1. We observed 22 amino acids in human seminal plasma after 3 hours in room temperature. They were Cysteic acid, taurine, Aspartic acid, Hydroxyproline, threonine, Serine, Asparagine, Glutamic acid, Glutamine, Proline, Glycine, Alanine, Cystine, Valine, Methionine, Isoleucine, Leucine, Tyrosine, Phenylalanine, Lysine, Histidine, and Arginine.2. Comparison between a healthy group and a group of infertility with normozoospermia revealed no differencies.3. This might be the first observation of Asparagine in human seminal plasma.