Increased mROS Generation Associates With Cardiovascular Risk in BioHEART ‐ CT PBMCs

Figures

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  FIGURE 1. Representative gating strategy for mitochondrial ROS production (MitoSOXTM Red) in freshly isolated patient‐derived PBMCs with or without CAD.

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  FIGURE 2. mROS production levels of PBMCs have an association with CAD severity. Univariate and adjusted (age, sex, smoking status, hypercholesteremia) standardized estimates of MitoSOX‐positive signature and CACS for each cohort in (A) PBMCs, (B) Lymphocytes, (C) Monocytes and (D) Statin naïve patients. N = 25 with CAD, N = 15 with No CAD. N = 8 with No CAD and Statin naïve, N = 13 with CAD and Statin naive.*p < 0.05.

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  FIGURE 3. mROS production levels of monocytes have an association with hypertension. Univariate and multivariable adjusted (age, sex, smoking status, hypercholesteremia, coronary artery disease status) logistic regression of MitoSOX‐positive signature and hypertension for each cohort in (A) PBMCs, (B) Lymphocytes, (C) Monocytes and (D) Statin naïve patients. N = 10 with Hypertension, N = 28 with No Hypertension. N = 5 with Hypertension and Statin naïve, N = 14 with No Hypertension and Statin naïve. *p < 0.05.

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  FIGURE 4. mROS production levels have no association with hypercholesteremia. Univariate and multivariable adjusted (age, sex, smoking status, hypertension, coronary artery disease status) logistic regression of MitoSOX‐positive signature and hypertension for each cohort in (A) PBMCs, (B) Lymphocytes, (C) Monocytes and (D) Statin naïve patients. N = 10 with Hypercholesteremia, N = 28 with No Hypercholesteremia. N = 5 with Hypercholesteremia and Statin naïve, N = 14 with No Hypercholesteremia and Statin naïve. *p < 0.05.

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  FIGURE 5. CCBE1 expression across immune cell types in patients with and without CAD using single cell RNA‐seq. (A) UMAP visualization of CCBE1 expression in matched patient peripheral blood mononuclear cells (PBMCs) from scRNA‐seq data. (B) CCBE1 expression in male CAD patient lymphocytes. (C) CCBE1 expression in male non‐CAD patient lymphocytes. Statistical comparisons were performed with Fisher's exact test.