Weanling male rats were fed purified diets containing 10% protein in the form of either casein or twice crystallized Fraction I protein from tobacco. Findings indicated that the average weight increment and PER (protein efficiency ratio) of rats fed the diet containing Fraction I protein from tobacco were significantly greater throughout a 28-day experimental period than that of rats fed the diet containing casein. No significant differences in hematological findings were noted between rats in the two dietary groups.
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Ershoff et al. (1978) studied this question.