Key result
LPS enhances superoxide anion formation and acid phosphatase activity in Atlantic cod macrophages versus controls.
Head kidney macrophages from Atlantic cod can be successfully isolated and cultured, demonstrating typical macrophage morphology, phagocytosis, and responsiveness to LPS stimulation.
LPS activates cod macrophages in vitro; hypothesis-generating for fish innate immunity, no practice implications yet.
Head kidney macrophages from Atlantic cod, Gadus morhua L., were isolated by density sedimentation and maintained under serum‐free conditions for up to one week. The cells adhered and spread well on glass and plastic, were highly phagocytic, and had typical macrophage morphology as shown by phase contrast and scanning and transmission electron microscopy. Histochemical studies with the light microscope showed that the macrophages were acid phosphatase and non‐specific esterase positive, and alkaline phosphatase and peroxidase negative. Compared with unstimulated control cells, LPS‐treated cells showed enhanced superoxide anion formation, as measured by the reduction of nitroblue tetrazolium, and increased levels of acid phosphatase activity.
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Sørensen et al. (1997) studied this question. LPS treatment vs. unstimulated control cells was evaluated on superoxide anion formation and acid phosphatase activity. LPS-treated Atlantic cod head kidney macrophages showed enhanced superoxide anion formation and increased levels of acid phosphatase activity compared with unstimulated control cells.
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