What question did this study set out to answer?

To outline standardized laboratory protocols for isolating murine macrophages from the peritoneal cavity and cultivating homogeneous populations from bone marrow progenitors.

September 1, 1994Current Protocols in Immunology

Isolation of Murine Macrophages

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To outline standardized laboratory protocols for isolating murine macrophages from the peritoneal cavity and cultivating homogeneous populations from bone marrow progenitors.
Isolation of murine macrophages from the peritoneal cavity under inflammatory and noninflammatory conditions.
Harvesting of bone marrow-derived progenitor cells followed by in vitro propagation and differentiation using CSFs or IL-3.
Provides procedures for harvesting peritoneal macrophages suitable for direct functional and immunological activation studies.
Yields homogeneous populations of mature macrophages propagated from immature bone marrow precursors for controlled experimental analysis.

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Implication

Laboratory protocol details isolation and culture methods for murine macrophages from tissue and marrow, highlighting standardized tools to examine immune activation.

Cite This Study

Fortier et al. (1994) studied this question.

synapsesocial.com/papers/6a7199863ce530166bc323a6 https://doi.org/10.1002/0471142735.im1401s11

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Implication

Laboratory protocol details isolation and culture methods for murine macrophages from tissue and marrow, highlighting standardized tools to examine immune activation.

Isolation of Murine Macrophages

Key Points

Discussion

Implication

Cite This Study

Isolation of Murine Macrophages

Key Points

Discussion

Implication

Cite This Study

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