What question did this study set out to answer?

To determine whether human peripheral blood basophils and eosinophils express functional Mas-related G protein-coupled receptor X2 (MRGPRX2).

January 31, 2020AllergyOpen Access

The pseudoallergen receptor MRGPRX2 on peripheral blood basophils and eosinophils: Expression and function

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Authors

BWBettina WediMedizinische Hochschule Hannover MGManuela GehringMedizinische Hochschule Hannover AKAlexander KappMedizinische Hochschule Hannover

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Implication

Laboratory study demonstrates functional expression of MRGPRX2 on basophils and eosinophils, indicating a broader role for this receptor in allergic and pseudoallergic reactions.

Key Points

To determine whether human peripheral blood basophils and eosinophils express functional Mas-related G protein-coupled receptor X2 (MRGPRX2).
Analyzed highly purified peripheral blood basophils and eosinophils from atopic and nonatopic donors using flow cytometry, immunocytochemistry, immunofluorescence, Western blot, and RT-PCR.
Evaluated functional capacity through calcium mobilization, fluorescent avidin-based degranulation, cytokine measurement, viability/apoptosis assays, and tricolor granulocyte activation testing.
Basophils and eosinophils, but not neutrophils, demonstrated significant MRGPRX2 expression that mediated calcium influx and concentration-dependent degranulation upon stimulation.
Receptor activation via anti-MRGPRX2 antibodies or ciprofloxacin prolonged cell survival, while receptor levels were upregulated by specific cytokines (such as IL-3 and IL-5) and grass pollen exposure in allergic subjects.

Cite This Study

Wedi et al. (2020) studied this question.

synapsesocial.com/papers/6ab1fed55eba7b26a37762f6 https://doi.org/10.1111/all.14213

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Implication

Laboratory study demonstrates functional expression of MRGPRX2 on basophils and eosinophils, indicating a broader role for this receptor in allergic and pseudoallergic reactions.

The pseudoallergen receptor MRGPRX2 on peripheral blood basophils and eosinophils: Expression and function

Discussion

Member takes

Implication

Key Points

Cite This Study

The pseudoallergen receptor MRGPRX2 on peripheral blood basophils and eosinophils: Expression and function

Discussion

Member takes

Implication

Key Points

Cite This Study

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