Key result
Tandem multiplex real-time PCR detects potential pathogens in 70% of children with acute respiratory symptoms.
Why the study?
A comprehensive, efficient method for detecting a wide range of respiratory pathogens in small sample volumes was needed to improve acute respiratory infection diagnosis.
Does a tandem multiplex real-time PCR technique efficiently detect multiple respiratory pathogens in children with acute respiratory symptoms?
Cross-Sectional (n=121)
Does a tandem multiplex real-time PCR technique efficiently detect multiple respiratory pathogens in children with acute respiratory symptoms?
Tandem multiplex real-time PCR is an efficient method for the rapid detection of multiple respiratory pathogens in clinical samples.
May support broader pathogen screening in pediatric respiratory illness; leaves open effects on management and outcomes.
This study used real-time PCR assays to screen small sample volumes for a comprehensive range of 35 respiratory pathogens. Initial thermocycling was limited to 20 cycles to avoid competition for reagents, followed by a secondary real-time multiplex PCR. Supplementary semi-nested human metapneumovirus and picornavirus PCR assays were required to complete the acute respiratory pathogen profile. Potential pathogens were detected in 85 (70%) of pernasal aspirates collected from 121 children with acute respiratory symptoms. Multiple pathogens were detected in 29 (24%) of those samples. The tandem multiplex real-time PCR was an efficient method for the rapid detection of multiple pathogens.
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Chidlow et al. (2009) conducted a cross-sectional in Acute respiratory symptoms (n=121). Tandem multiplex real-time PCR was evaluated on Detection of potential pathogens. Tandem multiplex real-time PCR detected potential pathogens in 70% of pernasal aspirates from children with acute respiratory symptoms, including multiple pathogens in 24% of samples.
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