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A two wavelength spectrophotometric method using tetramethylmurexide for assay of ionized calcium is described. Thermostating at 10° C and strict wavelength control have given high reproducibility and sensitivity. Calcium and indicator combine in a 1:1 ratio. A calcium ion activity coeffi cient curve as function of ionic strength is given. Stability constants for complexes of calcium and citrate, phosphate, sulphate, and lactate agreed with values obtained by more rigorous techniques.
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K. O. Pedersen (1970) studied this question.
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