C‐Terminal Incorporation of Bio‐Orthogonal Azide Groups into a Protein and Preparation of Protein–Oligodeoxynucleotide Conjugates by CuI‐Catalyzed Cycloaddition

Hooked up. Incorporation of a puromycin azide derivative into a protein C terminus has been achieved, in vitro. In combination with CuI-catalyzed cycloaddition, alkyl-modified oligodeoxynucleotides were specifically conjugated to the azide-activated polypeptide (see scheme). Self-assembly of protein–oligodeoxynuclotide conjugates to complementary oligodeoxynucleotide captures immobilized on an electrode microarray was demonstrated.