Key result
Palamneus gravimanus toxin selectively blocks human hKv1.1 potassium channels with a 10 nM affinity.
Why the study?
A novel peptide toxin from Palamneus gravimanus venom was isolated to investigate its selective blocking effect on human Kv1.1 potassium channels.
Effect estimate: Ki 10 nM
A novel peptide toxin from the Indian black scorpion selectively blocks human Kv1.1 channels, offering a potential tool for structural investigation of these channels.
A peptide toxin was isolated from the venom of Palamneus gravimanus, the Indian black scorpion, to block human Kv1.1 channels expressed in Xenopus laevis oocytes. A 4.5 kD peptide (toxin), as confirmed by SDS-PAGE, was purified to homogeneity by ion exchange chromatography using CM-Sephadex C-25 followed by Sephadex G-50 gel filtration. Palamneus gravimanus toxin (PGT) selectively blocks the human cloned voltage-gated potassium channel hKv1.1 in a two-electrode voltage-clamp (TEVC) technique. The results obtained indicate that the toxin blocks the hKv1.1 channel at a nanomolar concentration range (Ki value of 10 nM) of the peptide to the external side of the cell. The blockage seems to be voltage-dependent. Comparative structure of PGT (a 4.5 kD peptide) with BTK-2 suggests a close relationship; therefore this toxin can be employed to investigate the hKv1.1 channel structure.
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More et al. (2005) studied this question. Palamneus gravimanus toxin (PGT) was evaluated on Blockage of human cloned voltage-gated potassium channel hKv1.1 (Ki 10 nM). Palamneus gravimanus toxin (PGT) selectively blocked the human cloned voltage-gated potassium channel hKv1.1 with a Ki value of 10 nM.
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