Scatchard analyses demonstrate that at least three types of specific glucocorticoid binding occur in rat liver, epididymal fat pad, prostate, skeletal muscle and brain cerebral hemisphere cytosols in vitro. “Low” affinity (K ∼ 10− 10–810−9) cortisol binding appears to reflect at least partial plasma contamination, while “high” affinity (K ∼ 10–9–10–10) cortisol and dexamethasone binding occur at some common cytoplasmic sites. Numbers of specific “high” affinity glucocorticoid-binding sites in muscle, brain and epididymal fat pad appear to decrease progressively per unit weight and per unit protein, from sexual maturity at 2– months to at least 25 months of age. Prostate glands also decrease in this parameter between 13 and 25 months, but along with liver increase between sexual maturity and 13 months. Age-related glucocorticoid binding differences seem to reflect quantitative and/or qualitative changes in cytosol macromolecules since removal of small organic molecules prior to assessment of binding does not alter the ageassociated changes. (Endocrinology94: 82, 1974)
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George S. Roth (1974) studied this question.