Key result
EWS-E1AF fusion gene characterization aids Ewing's sarcoma diagnosis and contributes to oncogenesis.
Why the study?
Ewing's sarcoma expresses specific chimeric genes, but characterization of new fusion genes for diagnosis and understanding oncogenesis was needed.
The identification and characterization of the EWS-E1AF fusion gene provides a new molecular target for the diagnosis of Ewing's sarcoma.
May aid Ewing's sarcoma diagnosis via EWS-E1AF detection; leaves open its value as a therapeutic target.
Ewing's sarcoma, one of the most malignant tumors of children and young adults, expresses specific chimeric genes, e.g. EWS-FLI-1, EWS-ERG, EWS-ETV1 and EWS-FEV. In this paper, we extensively characterized a new fusion gene, EWS-EIAF by means of whole cDNA sequencing, RNA blot analysis, DNA blot analysis and chromosomal analysis, and showed it to be available for the diagnosis of Ewing's sarcoma and to participate in the oncogenesis of Ewing's sarcoma. Furthermore, we conducted a genetic analysis of Ewing family tumors in conjunction with immunohistochemical analysis and ultrastructural analysis. Our results demonstrate some limitations of both genetic analysis and histopathological analysis, and establish the relationship between neurogenic phenotypes and chimera genes.
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Urano et al. (1998) studied Ewing's sarcoma. Genetic analysis of EWS-E1AF fusion gene was evaluated. Characterization of the EWS-E1AF fusion gene showed it to be available for the diagnosis of Ewing's sarcoma and to participate in its oncogenesis.
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