Key result
111In-CHX-A"-DTPA-bevacizumab binds specifically to SKOV3 and LS174T tumor cells but not DU 145.
Why the study?
Radionuclide imaging using radiolabeled bevacizumab might be useful for selecting patients for anti-VEGF therapy, but specificity of binding to tumor cells needs evaluation.
111In-CHX-A"-DTPA-bevacizumab demonstrates cell line-dependent specific binding to VEGF, suggesting its potential utility as a scintigraphic imaging agent for selecting patients for anti-VEGF therapy.
Answers are grounded in this paper and cite their sources.
May guide anti-VEGF imaging development; leaves open clinical utility pending in vivo validation.
Bevacizumab is a humanized monoclonal antibody that binds to vascular endothelial growth factor (VEGF) and prevents tumor angiogenesis. Radionuclide imaging using radiolabeled bevacizumab might be useful for selection of patients for anti-VEGF therapy. This study describes preparation of a potential imaging agent, 111In-CHX-A"-DTPA-bevacizumab, and evaluation of specificity of its binding to three tumor cell lines, SKOV3, LS174T and DU 145. Bevacizumab was conjugated with CHX-A"-DTPA and radiolabeled with 111In with high yield and excellent stability. Specificity of cellular binding was examined by a saturation assay using 100-fold excess of non-radiolabeled antibody. SKOV3 and LS174T tumor cell lines showed significantly specific binding, while DU 145 cells did not showed any specific binding. The specific binding is dependent to type of cell lines, which it is important for selection of tumor model for scintigraphic imaging of the VEGF expression.
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Hosseinimehr et al. (2010) studied Tumor angiogenesis / VEGF expression. 111In-CHX-A"-DTPA-bevacizumab vs. Non-radiolabeled antibody was evaluated on Specificity of cellular binding to tumor cell lines. 111In-CHX-A"-DTPA-bevacizumab demonstrated significantly specific binding to SKOV3 and LS174T tumor cell lines, but not to DU 145 cells, indicating binding depends on the cell line type.
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