Three-Dimensional Cell Rotation With Fluidic Flow-Controlled Cell Manipulating Device

Three-dimensional positioning and orientation of a biological cell under a microscope is an essential step for cell injection and biopsy. The conventional manual cell manipulation and orientation relies on trial and error, with inherent disadvantages such as low efficiency, poor success rate, and inconsistent output. In this paper, we present a system capable of recognizing and adjusting the orientation of a Zebrafish (Danio rerio) embryo in real time with the use of multiple motion control devices. In addition, a custom-made embryo feeding channel and manipulating device was employed to perform cell manipulations in batches. Experimental results demonstrate the system's capability for fast embryo orientation (13 s/cell) with a 97.5% success rate of rotating a cell to a desired orientation about both y- and z-axis of the cell and a low in-plane rotation angle error of 0.5°.