Key result
Leukocyte lncRNA ENST00000593848.1 identifies acute aortic dissection with an AUC of ~0.79 in neutrophils.
Why the study?
The expression pattern and diagnostic potential of genome-scale leukocyte long noncoding RNAs in acute aortic dissection were not well characterized.
Do specific leukocyte long noncoding RNAs have diagnostic efficacy for acute aortic dissection?
Case-Control (n=40)
No
Do specific leukocyte long noncoding RNAs have diagnostic efficacy for acute aortic dissection?
Effect estimate: AUC 0.790
p-value: p=0.011
Specific leukocyte lncRNAs, particularly ENST00000593848.1, show potential as diagnostic biomarkers and anti-inflammatory regulators in acute aortic dissection.
May support ENST00000593848.1 as diagnostic biomarker for acute aortic dissection; leaves open prospective validation before clinical use.
Acute aortic dissection (AAD) is a disease with intima rupture and explosive inflammatory reaction. In this study, we detected the expression pattern of genome-scale leukocyte long noncoding RNAs (lncRNAs) in patients with AAD and explored the diagnostic efficacy and inflammatory mechanism. The expression levels of lncRNAs and mRNAs in 5 patients with AAD and 5 control cases were detected by microarray technology and were verified in 20 AAD cases and 10 control individuals. A total of 540 up- and 1078 down-regulated lncRNAs were significantly expressed between the two groups. Based on microarray analysis, confirmation through quantitative real-time polymerase chain reaction, and criteria for clinical relevance, three lncRNAs ENST00000430893.1, ENST00000536517.1, and ENST00000593848.1 were identified as potentially important diagnostic lncRNAs of AAD. Receiver operating characteristic curve (ROC curve) analysis showed that the area under curve (AUC) of ENST00000593848.1 was 0.790 (neutrophils, p < 0.05) and 0.725 (monocytes, p < 0.05). The corresponding target gene of ENST00000593848.1 was solute carrier family 8 member A1 (SLC8A1), which encodes a sodium calcium exchanger. Compared with transfection of no-load siRNA group, the Ca2+ levels, interleukin-6 (IL-6) levels, mRNA levels of extracellular regulated kinase (ERK) 1/2, and the activity of P-NF-κB p65 in THP-1 cells were increased after transfection of si-lncRNA (SILNC) (p < 0.05). Together, these results indicate that the three different lncRNAs ENST00000430893.1, ENST00000536517.1, and ENST00000593848.1 may play an anti-inflammatory role in patients with AAD, and ENST00000593848.1 is a promising candidate to further explore as a potential diagnostic and prognostic factor as well as for developing novel therapeutic strategies.
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A 2022 study conducted a case-control in Acute aortic dissection (n=40). Leukocyte long noncoding RNA ENST00000593848.1 expression vs. Healthy volunteers was evaluated on Diagnostic accuracy (Area Under the Curve) of ENST00000593848.1 in neutrophils (AUC 0.790, p=0.011). Expression levels of the leukocyte long noncoding RNA ENST00000593848.1 demonstrated significant diagnostic value for acute aortic dissection, yielding an AUC of 0.790 in neutrophils and 0.725 in monocytes.
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